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Related Experiment Video

Updated: May 28, 2026

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
07:53

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

Published on: April 1, 2016

Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper.

Barry R Lutz1, Philip Trinh, Cameron Ball

  • 1Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA. blutz@uw.edu

Lab on a Chip
|November 1, 2011
PubMed
Summary

Researchers developed two-dimensional paper networks (2DPNs) enabling multi-step reagent delivery for high-performance, easy-to-use point-of-care diagnostic devices. This innovation overcomes limitations of single-step paper tests.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Conventional paper-based diagnostic tests, like lateral flow tests, are typically restricted to single-step fluidic assays.
  • Achieving high performance in laboratory assays often necessitates multi-step protocols, which are challenging to implement in simple paper devices.

Purpose of the Study:

  • To develop a method for programming multi-step reagent delivery sequences in paper-based devices.
  • To enable the creation of point-of-care devices capable of performing complex, multi-step laboratory assays.

Main Methods:

  • Development of two-dimensional paper networks (2DPNs) using materials from lateral flow tests.
  • Reconfiguration of 2DPNs to incorporate multiple converging fluid inlets for controlled fluid arrival.

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Last Updated: May 28, 2026

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  • Implementation of a novel fluidic disconnection method using a shared buffer well and 2DPNs with legs of varying lengths to achieve timed reagent delivery sequences.

    • Main Results:

    • Demonstrated a method for programmed disconnection of fluid sources in 2DPNs.
    • Successfully controlled the timing of fluid delivery to a detection zone through sequential leg disconnection.
    • Validated the potential for 2DPNs to execute multi-step assay protocols.

    Conclusions:

    • The developed 2DPN approach enables programmable, multi-step reagent delivery, overcoming limitations of conventional paper-based tests.
    • This technology facilitates the translation of complex laboratory assays into user-friendly, high-performance point-of-care diagnostic devices.
    • The timed fluidic disconnection method offers a simple yet effective strategy for advanced fluid control in paper diagnostics.