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Updated: May 28, 2026
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Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
Published on: February 11, 2019
Yeast one-hybrid assays for gene-centered human gene regulatory network mapping
John S Reece-Hoyes1, A Rasim Barutcu, Rachel Patton McCord
1Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA. john.reece-hoyes@umassmed.edu
Nature Methods
|November 1, 2011
Abstract
None:
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
