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Updated: May 28, 2026
A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
Published on: June 30, 2023
Enhanced Y1H assays for Arabidopsis
Allison Gaudinier1, Lifang Zhang, John S Reece-Hoyes
1Department of Plant Biology and Genome Center, University of California Davis, Davis, California, USA.
Abstract:
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.
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