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Updated: May 28, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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Dual-polarization interferometry for quantification of small molecules using aptamers.

Veli Cengiz Ozalp1

  • 1Institute for Polymer Materials (POLYMAT), University of the Basque Country, Donostia-San Sebastian, Spain. cengizozalp@gmail.com

Analytical and Bioanalytical Chemistry
|November 1, 2011
PubMed
Summary

A new interferometry sensor accurately quantifies argininamide using aptamers. This method detects small molecule binding by measuring mass changes, not just thickness, achieving a 5 μM limit of detection.

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Area of Science:

  • Biosensing
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Accurate quantification of small molecules like argininamide is crucial in various scientific fields.
  • Existing detection methods may face challenges in real-time monitoring and sensitivity for low-mass analytes.
  • Interferometry offers a label-free approach for detecting molecular interactions on sensor surfaces.

Purpose of the Study:

  • To develop and validate an interferometry-based sensor for the sensitive detection and quantification of argininamide.
  • To investigate the use of aptamers immobilized on a silicone oxynitride surface for specific argininamide binding.
  • To explore the combined measurement of mass deposition and structural changes for enhanced analyte detection.

Main Methods:

  • Immobilization of DNA aptamers on a silicone oxynitride sensor surface via avidin-biotin linkage.

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  • Utilizing interferometry to monitor changes in mass and structure upon argininamide binding.
  • Calculating mass changes based on deposition and structural alterations detected by the sensor.
  • Determining the limit of detection (LOD) for argininamide quantification.

    • Main Results:

    • The aptamer layer formed a stable thin film (1.2 nm thickness) on the sensor surface.
    • Argininamide binding caused detectable mass changes without significant alterations in aptamer film thickness.
    • The developed sensor achieved a limit of detection of 5 μM for argininamide.
    • Real-time monitoring demonstrated high reliability and sensitivity in detecting argininamide concentration changes.

    Conclusions:

    • The interferometry-based sensor effectively quantifies argininamide by measuring mass changes, overcoming limitations of solely relying on structural data.
    • This approach provides a reliable and sensitive method for real-time monitoring of small mass analytes.
    • The combined measurement strategy enhances the detection capabilities for challenging analytes in biosensing applications.