Related Experiment Video

Updated: May 28, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

When second best is good enough: another probabilistic look at saturation mutagenesis.

Yuval Nov1

  • 1Department of Statistics, University of Haifa, Haifa, Israel. yuval@stat.haifa.ac.il

Applied and Environmental Microbiology
|November 1, 2011
PubMed
Summary

New criteria for saturation mutagenesis library size determination ensure finding top variants. This approach significantly reduces library size, saving resources while maintaining high-quality results for protein engineering.

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