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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: May 28, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Sensitive single-molecule protein quantification and protein complex detection in a microarray format.

Lee A Tessler1, Robi D Mitra

  • 1Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University, St. Louis, MO 63108, USA.

Proteomics
|November 1, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a digital antibody microarray for sensitive, simultaneous single-molecule protein detection. This breakthrough enables multiplexed proteomic profiling and biomarker discovery from complex biological samples.

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Last Updated: May 28, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

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Published on: January 7, 2019

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Published on: August 2, 2015

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Single-molecule protein analysis offers sensitive digital quantification for biological studies and clinical biomarker detection.
  • Current single-molecule platforms are limited to analyzing one protein at a time.
  • Conventional antibody microarrays allow multiplexing but lack sensitivity and quantitative accuracy due to ensemble averaging.

Purpose of the Study:

  • To develop a single-molecule protein assay in a microarray format for multiplexed analysis.
  • To achieve high sensitivity and broad dynamic range for protein quantitation.
  • To demonstrate the utility of digital antibody microarrays for proteomic profiling and biomarker detection.

Main Methods:

  • Implementation of an ultra-low background surface for single-molecule imaging.
  • Utilizing hybrid digital-analog quantification for a wide dynamic range.
  • Development of a microarray format for parallel single-molecule protein detection.

Main Results:

  • Achieved a low femtomolar limit of detection and four orders of magnitude dynamic range.
  • Successfully measured p53 and MDM2 levels in parallel from crude cell lysate.
  • Detected the p53-MDM2 protein complex in cell lysate using the single-molecule microarray.

Conclusions:

  • Demonstrated the feasibility of a digital antibody microarray for multiplexed, single-molecule protein analysis.
  • The developed platform bridges the gap between single-plex and highly multiplexed protein detection methods.
  • This technology holds significant promise for advancing proteomic profiling and clinical biomarker discovery.