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Related Experiment Video

Updated: May 28, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Alternative transcript initiation and splicing as a response to DNA damage.

Carl N Sprung¹, Jason Li, Daniel Hovan

¹Centre for Innate Immunity and Infectious Disease, Monash Institute for Medical Research, Monash University, Clayton, Victoria, Australia. carl.sprung@monash.edu

|November 1, 2011

Summary

Ionizing radiation (IR) exposure alters gene expression in human cells. This study reveals previously unknown alternative transcripts and gene regulation patterns at the exon level, offering new insights into DNA damage and stress responses.

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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

Molecular Biology
Genomics
Radiation Biology

Background:

Humans encounter ionizing radiation (IR) from various sources, impacting cellular processes.
While IR affects transcription, genome-wide understanding of alternative transcription in response to IR remains limited.

Purpose of the Study:

To comprehensively characterize radiation-induced transcriptional changes at the exon level in human cells.
To identify novel alternative transcripts and gene regulation mechanisms in response to IR.

Main Methods:

Utilized exon arrays for genome-wide analysis of transcriptional expression products following IR exposure.
Investigated dose-response and time-course kinetics of gene expression changes.

Main Results:

Discovered previously uncharacterized alternative transcripts preferentially occurring after IR exposure.
Identified numerous genes exhibiting alternative transcription initiation in response to IR.
Observed co-ordinately regulated gene clusters at specific chromosomal loci.

Conclusions:

Provides the first genome-wide perspective on the exon-level transcriptional response to ionizing radiation.
Highlights alternative transcripts as a significant mechanism for cellular response to DNA damage and stress.