Related Experiment Video
Updated: May 28, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
Published on: January 9, 2017
Low-coherence dynamic light scattering and its potential for measuring cell dynamics
Katsuhiro Ishii1, Toshiaki Iwai
1The Graduate School for the Creation of New Photonics Industries, 1955-1 Kurematsucho, Nishi-Ku, Hamamatsu, 4311202, Japan. ishii@gpi.ac.jp
Abstract:
Fluorescence correlation spectroscopy is a powerful technique for studying the structures and dynamics of living cells. Dynamic light scattering (DLS) is also used to study dynamic characteristics and it has the potential to measure cell dynamics. However, it is difficult to apply DLS to highly scattering media. In this article, we review low-coherence dynamic light scattering (LC-DLS). It strongly suppresses the influence of multiple scattering and has a greater potential for measuring cell dynamics than conventional DLS. The properties of LC-DLS are described theoretically and experimentally. Measurement of the diffusion coefficients of macromolecules in turbid media and interparticle and molecular interactions by LC-DLS is demonstrated.
Related Concept Videos
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Three-Dimensional Microscopy in Microbiology
Two-Dimensional Microscopy in Microbiology

