Related Experiment Video
Updated: May 28, 2026

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
Published on: June 15, 2014
Silage produces biofuel for local consumption
Hiroko K Kitamoto1, Mitsuo Horita1, Yimin Cai2,3
1National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
This study presents a decentralized bio-ethanol production system using solid-state fermentation of whole crops. The novel method efficiently produces bio-ethanol and a cattle feed byproduct with no waste.
Area of Science:
- Agricultural Science
- Biotechnology
- Chemical Engineering
Background:
- Traditional bio-ethanol production involves centralized factories, leading to nutrient loss during biomass processing.
- A decentralized system is needed for farm-level production, integrating silage methods with simultaneous saccharification and fermentation (SSF).
Purpose of the Study:
- To develop a decentralized, waste-free bio-ethanol production system using solid-state fermentation (SSF) of whole crops.
- To simultaneously produce bio-ethanol and cattle feed from biomass, incorporating traditional silage techniques.
Main Methods:
- Harvested fresh biomass (forage rice) was ensiled with biomass-degrading enzymes and yeast in a solid-state culture.
- Simultaneous saccharification and fermentation (SSF) reactions were induced in hermetically sealed containers.
- Ethanol was recovered on-site from the vapor of heated fermented products.
Main Results:
- Efficient induction of parallel SSF reactions was achieved in a laboratory-scale model system.
- A yield of 6.4 wt.% ethanol (169 g/kg dry matter) was produced from forage rice after 20 days.
- 74% of the produced ethanol was recovered via vapor distillation, with significant reduction in organic cellular and fiber content.
Conclusions:
- Efficient bio-ethanol production from non-sterilized whole rice plants using laboratory-scale SSF is feasible.
- Further research is required for scaling up, optimizing ethanol recovery, and validating the residue as cattle feed.
Related Concept Videos
Biofuels
Microbes in the Production of Fermented Foods
Environmental Applications of Microorganisms
Production of Organic Acids
Microbes in Food Production
Production of Alcohol

