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Updated: May 28, 2026
05:58
In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
Published on: September 6, 2024
Electron microscopy studies of nucleosome remodelers
1Department of Molecular and Cellular Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA. aleschziner@mcb.harvard.edu
Current Opinion in Structural Biology
|November 2, 2011
Summary
ATP-dependent chromatin remodelers alter nucleosome structure using ATP. Structural data from electron microscopy provides functional insights into these essential protein complexes, despite challenges in sample preparation.
Area of Science:
- Biochemistry
- Molecular Biology
- Structural Biology
Background:
- ATP-dependent chromatin remodeling complexes regulate chromatin dynamics.
- Understanding their mechanism and regulation is limited by scarce structural data.
Purpose of the Study:
- To review structural data obtained through electron microscopy.
- To provide functional insights into chromatin remodeling complexes.
Main Methods:
- Review of existing electron microscopy (EM) studies.
- Single-particle analysis of remodeling complexes.
Main Results:
- EM has enabled structural characterization of several remodeling complexes.
- Structural data offers functional insights into remodeling mechanisms.
Conclusions:
- Electron microscopy is a valuable tool for studying challenging remodeling complexes.
- Further structural studies will enhance understanding of chromatin dynamics regulation.
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