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Related Experiment Video

Updated: May 28, 2026

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding
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Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

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3D thermoplastic elastomer microfluidic devices for biological probe immobilization.

Daniel Brassard1, Liviu Clime, Kebin Li

  • 1Industrial Materials Institute, National Research Council, Boucherville, QC, Canada. Daniel.Brassard@imi.cnrc-nrc.gc.ca

Lab on a Chip
|November 2, 2011
PubMed
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We developed a novel microfluidic device using thermoplastic elastomer membranes for high-throughput biological probe immobilization. This cost-effective method enables precise patterning of DNA and protein arrays on solid supports.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Materials Science

Background:

  • Microfluidics offers high-resolution biological probe patterning but faces challenges in mass production and 3D channel fabrication.
  • Current techniques struggle with adapting prototyping methods for cost-effective, large-scale manufacturing of microfluidic biological immobilization tools.

Purpose of the Study:

  • To present a novel fabrication method for microfluidic devices enabling cost-effective, high-throughput biological probe immobilization.
  • To demonstrate a 3D microfluidic device for precise patterning of isolated biological spots suitable for mass production.

Main Methods:

  • Fabrication of thin thermoplastic elastomer membranes with microscopic through-holes via hot embossing, compatible with high-throughput manufacturing.
  • Design and optimization of a 3D microfluidic channel network using Lattice-Boltzmann simulations to utilize capillary action for liquid distribution.

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Last Updated: May 28, 2026

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding
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Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

Published on: June 27, 2014

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Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics

Published on: September 10, 2018

Microfabricated Platforms for Mechanically Dynamic Cell Culture
15:21

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  • Integration of membranes into 1 cm² devices for creating 10x10 arrays of 50x50 μm² isolated spots.

    • Main Results:

    • Successful fabrication of integrated 3D microfluidic devices from thermoplastic elastomer membranes using a mass-production compatible hot-embossing process.
    • Demonstrated immobilization of up to 96 different biological probes in isolated spots using capillary action-driven liquid distribution.
    • Achieved highly specific DNA and protein array patterning on thermoplastic substrates with excellent spot definition.

    Conclusions:

    • The developed microfluidic device fabrication method overcomes limitations in mass production for biological probe immobilization.
    • The 3D microfluidic system enables precise, high-throughput patterning of biological arrays, suitable for various applications including diagnostics and research.
    • This approach offers a cost-effective and scalable solution for creating advanced microarrays.