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Titanium preferential binding sites in human serum transferrin at physiological concentrations.

Yoana Nuevo-Ordoñez¹, M Montes-Bayón, E Blanco González

¹Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Oviedo, Spain.

Metallomics : Integrated Biometal Science

|November 2, 2011

Summary

Titanium (Ti) in patients with intramedullary nails binds to serum transferrin (Tf) specifically in the N-lobe. This binding occurs in vivo and is unaffected by sialic acid levels, suggesting a cellular entry pathway for titanium.

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Area of Science:

Biochemistry
Metalloprotein Chemistry
Biomaterials Science

Background:

Serum transferrin (Tf) is crucial for iron metabolism, binding iron and other metal ions.
Titanium (Ti) is used in intramedullary nails, and its binding to Tf in vivo is poorly understood.
Previous studies show Tf binds Ti tightly in vitro, but physiological relevance is unclear.

Purpose of the Study:

To investigate the in vivo chemical association of titanium to serum transferrin in patients with intramedullary titanium nails.
To determine the specific binding site of titanium on transferrin.
To explore the influence of sialic acid on titanium-transferrin binding.

Main Methods:

Elemental mass spectrometry using double focusing inductively coupled plasma (DF-ICP-MS) for detection.
Chromatographic separation techniques with varying gradient conditions to analyze Ti/Fe-Tf forms.
Neuraminidase (sialidase) incubation to assess the role of sialic acid in Tf.

Main Results:

Titanium in human serum from patients with intramedullary nails is uniquely associated with the N-lobe of transferrin.
Titanium binding affinity to transferrin remains similar for both native and monosialylated Tf.
Binding of Ti to Tf occurs specifically in the N-lobe, irrespective of sialic acid content.

Conclusions:

In vivo titanium from implants uniquely binds to the N-lobe of serum transferrin.
Sialic acid content does not significantly affect titanium binding to transferrin.
The Fe(C)Ti(N)-Tf complex may facilitate cellular titanium uptake via transferrin receptors.