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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: May 28, 2026

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

AFM to study bio/nonbio interactions.

Holger Schönherr¹

¹Department of Physical Chemistry I, University of Siegen, Siegen, Germany. schoenherr@chemie.uni-siegen.de

Methods in Molecular Biology (Clifton, N.J.)

|November 2, 2011

Summary

This study presents a method for attaching biomolecules to gold surfaces to measure bio/nonbio interactions using atomic force microscopy (AFM). This technique allows for the quantitative analysis of forces between biological and nonbiological materials.

Area of Science:

Biophysics
Surface Chemistry
Biomaterials Science

Background:

Studying interactions between biomolecules and nonbiological entities is crucial in various scientific fields.
Existing methods for analyzing these interactions can be limited in scope or quantitative precision.

Purpose of the Study:

To describe a versatile method for immobilizing proteins and other biomolecules onto gold surfaces.
To enable the study of forces between biomolecules and nonbiological entities using atomic force microscopy (AFM).

Main Methods:

Utilizing reactive self-assembled monolayers on gold substrates for biomolecule immobilization.
Immobilizing biomolecules on both flat gold surfaces and gold-coated AFM probe tips.
Performing AFM experiments in liquid environments and conducting quantitative analysis of adhesive forces.

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Main Results:

Successful immobilization of biomolecules on gold surfaces and AFM probe tips.
Demonstration of quantitative measurement of adhesive forces between biomolecules and nonbiological entities.
Establishment of a versatile platform for bio/nonbio interaction studies.

Conclusions:

The described approach provides a robust and adaptable tool for investigating bio/nonbio interactions.
This method facilitates the experimental addressing of a wide range of problems in biomolecular interaction analysis.
The technique is valuable for researchers in biophysics, materials science, and nanotechnology.