Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Detecting and reconstructing breakage-fusion-bridge cycles from long-read sequencing using BFBArchitect.

Bioinformatics (Oxford, England)·2026

Same author

Senescent Human Liver Endothelial Cells Mediate CD4<sup>+</sup> T Cell Recruitment via ICOSL.

Immunology·2026

Same author

Identification of Cyclin L1 as a Host Factor Regulating Hepatitis B Virus Replication.

Viruses·2026

Same author

Author Correction: Towards a reference cell atlas of liver diversity over the human lifespan.

Nature reviews. Gastroenterology & hepatology·2025

Same author

Towards a reference cell atlas of liver diversity over the human lifespan.

Nature reviews. Gastroenterology & hepatology·2025

Same author

Developing risk stratification strategies and biomarkers for recurrent hepatocellular carcinoma.

Clinical and translational medicine·2025

Search research articles

Related Experiment Video

Updated: May 28, 2026

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

A systematic screen for micro-RNAs regulating the canonical Wnt pathway.

Roman Anton¹, Sujash S Chatterjee, Julia Simundza

¹Cancer Institute/Department of Pharmacology, New York University Langone Medical Center, New York University, New York, New York, United States of America.

|November 2, 2011

Summary

MicroRNAs (miRs) that regulate the Wnt pathway were identified. Some miRs, like miR-1 and miR-25, inhibit cancer cell growth, suggesting potential diagnostic and therapeutic uses in Wnt-related diseases.

Related Experiment Videos

Last Updated: May 28, 2026

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

Area of Science:

Molecular Biology
Cancer Research
Genetics

Background:

MicroRNAs (miRs) and the Wnt pathway are frequently dysregulated in human cancers.
These molecules play critical roles in cancer initiation and progression.

Purpose of the Study:

To identify miRs that modulate the canonical Wnt pathway activity.
To investigate the potential of Wnt-modulating miRs as cancer biomarkers and therapeutics.

Main Methods:

A cell-based overexpression screen of 470 miRs in HEK293 cells.
Literature survey and epistasis-based functional validation of candidate miRs.
Assessment of miR effects on cell proliferation, viability, and Wnt pathway reporter gene expression in cancer cells and organoids.

Main Results:

38 candidate miRs were identified that either activate or repress the Wnt pathway.
Wnt-repressing miRs were found to be anti-oncomiRs downregulated in cancers, while Wnt-activating miRs were oncomiRs upregulated during tumorigenesis.
miR-1, miR-25, and miR-613 were validated as Wnt pathway repressors, with miR-25 acting at the β-catenin level and miR-1/miR-613 acting upstream.
miR-1 and miR-25 inhibited proliferation and viability in colon cancer cells.
miR-1 expression reduced Wnt pathway activity in mouse mammary organoids.

Conclusions:

Wnt-modulating miRs, such as miR-1 and miR-25, can inhibit cancer cell proliferation and viability.
These miRs represent potential diagnostic and therapeutic tools for Wnt-dependent diseases, including cancer.