Related Experiment Video
Updated: May 28, 2026
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Published on: December 21, 2016
[Chromosome damage of human bronchial epithelial cells induced by glycidyl methacrylate]
Quankai Wang1, Ying Li, Lin Dong
1National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China. kylewang@sina.com
Objective:
To observed the chromosome damage of human bronchial epithelial cells induced by glycidyl methacrylate (GMA).
Methods:
Chromosome aberration analysis of human bronchial epithelial cells treated with GMA at different dosages (4, 8, 12, 16 and 20 microg/ml), times (1, 2 and 3 times), and phases (10th, 30th genetation) was detected.
Results:
In the dosages range from 4 to 20 microg/ml, the aberration rates (3%, 6%, 7%, 11% and 14%) were demonstrated increasingly with the increase of exposure doses, and dose-effect relationship was found. Significant differences were observed when treated with GMA three times (6%, 7% and 10%). Structure aberrations were found in the transformed 10th-generation cells, while number aberrations were mainly manifested in the transformed 30th-generation cells.
Conclusion:
The chromosome aberration can be induced by GMA in the human bronchial epithelial cells, from the structure aberration at the beginning to the lack of normal nuclear style.
Related Concept Videos
01:24Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features
01:20Chronic Obstructive Pulmonary Disease-II: Pathophysiology
Chronic Inflammation