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Updated: May 28, 2026

Chicken Embryo as an In Vivo Model to Revive Viable but Non-Culturable Pathogens
Published on: May 30, 2025
[Research progress of VBNC bacteria--a review]
Linxian Ding1, Xiaomei Su, Akira Yokota
1College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China. linxian@zjnu.cn
The viable but non-culturable (VBNC) state allows bacteria to survive without growth on standard media. This study explores VBNC formation, resuscitation, and its implications in bacterial research.
Area of Science:
- Microbiology
- Bacterial Physiology
Context:
- The viable but non-culturable (VBNC) state represents a survival strategy for bacteria under stress.
- Understanding VBNC mechanisms is crucial for microbial ecology and food safety.
Purpose:
- To elucidate the formation, characteristics, and resuscitation of the VBNC state in bacteria.
- To review the research significance and application prospects of VBNC bacteria.
Summary:
- This article details the formation mechanisms, variations, and resuscitation methods of the VBNC state.
- It presents 10 years of research on bacterial VBNC states, covering culturability, phylogeny, and functions.
Impact:
- Provides insights into bacterial survival strategies and stress responses.
- Highlights the potential applications of VBNC bacteria in biotechnology and environmental science.
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