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Updated: May 28, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
Published on: November 3, 2011
Protein structural domains: definition and prediction
Iakes Ezkurdia1, Michael L Tress1
1Spanish National Cancer Research Centre (CNIO)-Structural Biology and Biocomputing Programme, Madrid, Spain.
Abstract:
Recognition and prediction of structural domains in proteins is an important part of structure and function prediction. This unit lists the range of tools available for domain prediction, and describes sequence and structural analysis tools that complement domain prediction methods. Also detailed are the basic domain prediction steps, along with suggested strategies for different protein sequences and potential pitfalls in domain boundary prediction. The difficult problem of domain orientation prediction is also discussed. All the resources necessary for domain boundary prediction are accessible via publicly available Web servers and databases and do not require computational expertise.
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