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Related Experiment Video

Updated: May 28, 2026

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
05:46

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Published on: March 7, 2017

A high throughput, interactive imaging, bright-field wound healing assay.

Michael D Zordan1, Christopher P Mill, David J Riese

  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|November 3, 2011
PubMed
Summary

A new high-throughput wound healing assay uses laser ablation and image analysis for faster, more accurate cell migration studies. This automated method enables efficient drug screening by quantifying wound closure rates.

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Area of Science:

  • Cell biology
  • Biotechnology
  • Medical imaging

Background:

  • The wound healing assay is crucial for studying cell migration.
  • Traditional methods lack throughput and quantitative analysis.
  • Need for automated, high-throughput cell migration assays.

Purpose of the Study:

  • To develop a high-throughput wound healing assay using the LEAP™ instrument.
  • To enable quantitative data analysis of cell migration.
  • To improve speed and accuracy for drug screening.

Main Methods:

  • Utilized the LEAP™ instrument for laser ablation to create reproducible wounds in 96-well plates.
  • Employed bright-field imaging to capture cell migration over time.
  • Developed a custom texture segmentation algorithm for quantitative wound area analysis.

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Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
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Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

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Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
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Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

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Related Experiment Videos

Last Updated: May 28, 2026

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
05:46

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Published on: March 7, 2017

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
07:32

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
08:13

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

Main Results:

  • Laser ablation created reproducible wounds with <10% area variation.
  • The automated assay provided faster and more accurate image analysis than traditional methods.
  • Confirmed neuregulin-2β dose-dependently increased MCF7 cell wound healing rates.

Conclusions:

  • The developed automated wound healing assay significantly enhances speed and accuracy.
  • This high-throughput method is suitable for quantitative cell migration studies and drug screening.
  • Laser ablation and advanced image analysis offer a robust platform for cell motility research.