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Updated: May 28, 2026

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
07:49

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Published on: August 27, 2011

Preparation of Drosophila eye specimens for transmission electron microscopy

Tanya Wolff

    Cold Spring Harbor Protocols
    |November 3, 2011
    PubMed

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    Preparation of Samples for Electron Microscopy01:20

    Preparation of Samples for Electron Microscopy

    To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...