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Oral rehydration salt-liquid as an alternative storage medium--a preliminary study
P Subramaniam1, U Eswara, K L Girish Babu
1Department of Pedodontics And Preventive Dentistry, The Oxford Dental College, Hospital And Research Centre, Bommanahalli, Hosur Road, Bangalore-560068, Karnataka, India. drpriyapedo@yahoo.com
Objective:
To assess the efficacy of Oral Rehydration Salt-Liquid (ORS-L) in the maintenance of the viability of Periodontal ligament cells (PDL).
Materials And Method:
Twenty freshly extracted teeth were used for this study. They were then randomly divided into 3 groups: Positive control group--5 teeth which were immediately subjected to collagenase assay, without immersing in ORS-L; Negative control group--5 teeth with an extra oral dry time of 24 hours, followed by subjecting to collagense assay without immersing in ORS-L and Test group (ORS-L)--10 teeth with an extra oral dry period of 30 minutes, followed by immersion in ORS-L for a period of 45 minutes and then subjected to collagenase assay. The mean number of viable PDL cells were counted on a hemocytometer under 20X magnification.
Results:
The mean number of viable PDL cells was highest in the positive control group. In comparison to the negative control group, the test group showed a higher number of viable PDL cells.
Conclusion:
The study found that ORS-L was an effective solution in maintaining the viability of PDL cells.
