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Updated: May 28, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte.

Yifan Wang¹, Ruoyu Zhan, Tianhu Li

¹Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore, Singapore 117567, Singapore.

Langmuir : the ACS Journal of Surfaces and Colloids

|November 4, 2011

Summary

This study introduces a novel visual assay for detecting single nucleotide polymorphisms (SNPs) using DNA ligase, rolling circle amplification, and fluorescent polymers. The assay achieves a 1 nM detection limit for SNPs, offering improved visual contrast and sensitivity.

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

Molecular Biology
Biotechnology
Analytical Chemistry

Background:

Single nucleotide polymorphisms (SNPs) are key genetic variations influencing disease susceptibility and drug response.
Accurate and sensitive detection of SNPs is crucial for genetic diagnostics and personalized medicine.
Existing SNP detection methods often require complex instrumentation and are time-consuming.

Purpose of the Study:

To develop a simple, visual assay for sensitive and selective detection of single nucleotide polymorphisms (SNPs).
To leverage enzymatic reactions and fluorescent polymers for a colorimetric SNP detection system.
To establish a low detection limit for visual SNP identification.

Main Methods:

Utilized Escherichia coli (E. coli) DNA ligase for selective circularization of perfectly matched DNA targets.
Employed rolling circle amplification (RCA) with Phi29 enzyme to amplify target DNA sequences.
Incorporated cationic conjugated polymers (PFBT(20) and PFP) and FAM-labeled peptide nucleic acid (PNA) for signal generation and visual detection.

Main Results:

Achieved selective SNP detection through DNA ligase-mediated circularization.
Demonstrated distinct color changes (blue-whitish to yellow) upon DNA amplification via RCA.
Further enhanced visual contrast and achieved a 1 nM detection limit for target DNA using PNA-DNA hybridization and PFP-mediated energy transfer, resulting in a green fluorescence.

Conclusions:

The developed assay provides a simple, sensitive, and selective visual method for SNP detection.
The combination of enzymatic amplification and fluorescent polymers offers a cost-effective alternative to traditional SNP analysis.
This assay has potential applications in point-of-care diagnostics and high-throughput genetic screening.