Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of the Electrophilic Allergen Screening Assay for Detection of Key Event 1 of the Skin Sensitization Adverse Outcome Pathway.

Toxics·2026
Same author

A single-cell multi-omics assay for simultaneous measurement of vector copy number and protein expression in CAR T cells.

Molecular therapy. Advances·2026
Same author

Melanoma and Other Melanistic Lesions in Brown Bullhead Ameiurus nebulosus From Waterbodies in the Northeastern United States and Canada: Identification of Risk Factors.

Journal of fish diseases·2026
Same author

Tissue metal concentrations and toxicity in wild turkeys following chronic exposure to mining-contaminated soils in southeast Missouri, USA.

Ecotoxicology (London, England)·2026
Same author

Review of potential mitigation methodologies for existing concrete structures containing pyrrhotite-bearing aggregates.

Journal of building engineering·2026
Same author

Chronic exposure to waterborne nickel significantly reduced growth of juvenile crayfish (Faxonius virilis).

Ecotoxicology (London, England)·2026

Related Experiment Video

Updated: May 28, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

NIST gold nanoparticle reference materials do not induce oxidative DNA damage.

Bryant C Nelson1, Elijah J Petersen, Bryce J Marquis

  • 1National Institute of Standards and Technology, Material Measurement Laboratory, Gaithersburg, MD 20899, USA. bryant.nelson@nist.gov

Nanotoxicology
|November 4, 2011
PubMed
Summary

The National Institute of Standards and Technology developed gold nanoparticle (AuNP) reference materials. These AuNPs showed no significant genotoxicity, suggesting their suitability as negative controls in nanotoxicology studies.

More Related Videos

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy
10:51

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy

Published on: August 28, 2013

Related Experiment Videos

Last Updated: May 28, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy
10:51

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy

Published on: August 28, 2013

Area of Science:

  • Nanotoxicology
  • Biomaterials Science
  • Genetics

Background:

  • A critical gap in nanotoxicology is the absence of well-characterized nanoparticle reference materials for controls.
  • Standardized reference materials are essential for reproducible and reliable genotoxicity testing.

Purpose of the Study:

  • To evaluate the genotoxicity of gold nanoparticle (AuNP) reference materials developed by the National Institute of Standards and Technology (NIST).
  • To determine the suitability of these AuNPs as negative controls in nanotoxicology studies.

Main Methods:

  • Genotoxicity testing of NIST AuNPs (10, 30, and 60 nm) using HepG2 cells and calf-thymus DNA.
  • Quantification of four specific oxidatively-modified DNA lesions using liquid chromatography/tandem mass spectrometry.
  • Detection of free radicals via electron paramagnetic resonance spectroscopy.

Main Results:

  • No significantly elevated, dose-dependent DNA damage was observed at AuNP concentrations up to 0.2 μg/ml.
  • Free radical generation was not detected by electron paramagnetic resonance spectroscopy.
  • The tested NIST AuNPs did not induce detectable genotoxic effects under the experimental conditions.

Conclusions:

  • NIST gold nanoparticles demonstrate potential as reliable negative-control reference materials for in vitro and in vivo genotoxicity assessments.
  • These reference materials can enhance the reproducibility and validity of nanoparticle safety evaluations.
  • The findings support the use of characterized nanomaterials to standardize nanotoxicology research.