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Related Experiment Video

Updated: May 28, 2026

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

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Published on: August 12, 2018

Oxidative stress and leaf senescence.

Hatami Gigloo Sedigheh1, Mahdi Mortazavian, Dariush Norouzian

  • 1Pasteur Institute of Iran (Research & Production complex), Department of Research and Development, 25th km Tehran Karaj Highway, Tehran, Iran. mghorbani@irimc.org.

BMC Research Notes
|November 4, 2011
PubMed
Summary

Oxidative stress from paraquat damages Rubisco, the key enzyme in photosynthesis, by affecting CO2 assimilation. This deactivation signals Rubisco degradation during plant senescence.

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Published on: August 26, 2018

Area of Science:

  • Plant Biology
  • Biochemistry
  • Photosynthesis Research

Background:

  • Plant senescence involves genetically controlled cell death.
  • Oxidative stress, particularly from paraquat, induces reactive oxygen species (ROS) in chloroplasts.
  • Plants reallocate nutrients during senescence and stress, with Rubisco being a major protein affected.

Purpose of the Study:

  • To evaluate the impact of ROS on Rubisco during plant senescence and oxidative stress.
  • To identify key photosynthetic factors indicative of Rubisco damage.

Main Methods:

  • Measured photosynthesis factors: net photosynthesis rate (Pn), stomatal conductance (G), evaporation rate (E), intracellular CO2 concentration (Ci), fluorescence, and total protein.
  • Assessed these factors across three developmental stages in paraquat-treated plants.

Main Results:

  • Intracellular CO2 concentration (Ci) showed the highest correlation with Rubisco damage.
  • Paraquat-induced ROS primarily impacts Rubisco activity and CO2 assimilation.
  • Correlation between photosynthesis rate and total protein was significantly lower than with Ci.

Conclusions:

  • ROS significantly affects Rubisco activity early in oxidative stress, increasing susceptibility to proteases.
  • Rubisco deactivation serves as an initial signal for its subsequent degradation.
  • Understanding Rubisco's response to stress is crucial for plant survival and nutrient reallocation.