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Related Experiment Videos

Eukaryotic DNA ligases.

D D Lasko1, A E Tomkinson, T Lindahl

  • 1Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts., Great Britain.

Mutation Research
|September 1, 1990
PubMed
Summary
This summary is machine-generated.

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Eukaryotic DNA ligases, crucial for DNA repair, have been purified and characterized. Studies reveal sequence similarities among yeast and viral DNA ligases, with altered DNA ligase I activity noted in Bloom's syndrome cell lines.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Eukaryotic DNA ligases are essential enzymes involved in DNA replication and repair.
  • Two distinct DNA ligases, DNA ligase I and DNA ligase II, have been identified in mammalian cells.
  • DNA ligases have also been found in other organisms, including Drosophila melanogaster, yeast, and viruses.

Purpose of the Study:

  • To review recent studies on eukaryotic DNA ligases.
  • To characterize purified DNA ligase I and II from mammalian cells.
  • To investigate the genetic and biochemical properties of DNA ligases across different species.

Main Methods:

  • Purification of mammalian DNA ligase I and II to homogeneity.
  • Biochemical characterization of purified enzymes.

Related Experiment Videos

  • Cloning and sequencing of DNA ligase genes from yeast and vaccinia virus.
  • Amino acid sequence comparison.

    • Main Results:

    • Mammalian DNA ligase I and II were purified and biochemically characterized.
    • Distinct DNA ligases were identified in Drosophila melanogaster embryos.
    • DNA ligase genes from Schizosaccharomyces pombe, Saccharomyces cerevisiae, and vaccinia virus were cloned and sequenced.
    • Proteins showed significant amino acid sequence identity (30% among the three, 53% between the two yeast enzymes).
    • Altered DNA ligase I activity was observed in cell lines from Bloom's syndrome patients.

    Conclusions:

    • Eukaryotic DNA ligases share conserved features, suggesting a common evolutionary origin.
    • Further research is needed to establish a causal link between DNA ligase I deficiency and Bloom's syndrome.