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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
Published on: May 24, 2012
The inside out of lentiviral vectors
Stéphanie Durand1,2,3,4, Andrea Cimarelli1,2,3,4
1Department of Human Virology, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon, France.
Abstract:
Lentiviruses induce a wide variety of pathologies in different animal species. A common feature of the replicative cycle of these viruses is their ability to target non-dividing cells, a property that constitutes an extremely attractive asset in gene therapy. In this review, we shall describe the main basic aspects of the virology of lentiviruses that were exploited to obtain efficient gene transfer vectors. In addition, we shall discuss some of the hurdles that oppose the efficient genetic modification mediated by lentiviral vectors and the strategies that are being developed to circumvent them.
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