Related Experiment Video
Updated: May 27, 2026

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
Published on: February 14, 2016
[Studies on SNP and genomic imprinting of the PEG1 gene in swine]
Li Li1, Tong Jia, Tong-Shan Wu
1Guangdong Provincial Key Laboratory ofAgro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agriculture University, Guangzhou 510642, China. lili007@scau.edu.cn
Abstract:
PEG1 affects animal embryo growth and maternal behavior. The paternal allele-specific expression of PEG1 was reported in most animal species, but the expressive pattern of PEG1 was not clear in piglets born. In order to investigate the imprinting pattern of PEG1 in pig, 166 samples were used to SNP of PEG1 from Landrace, Yorkshire, and Lantang breeds by PCR-SSCP. Single nucleotide polymorphism (SNP) of PEG1 in exon 12 was identfied. The genotype frequency and the expressive pattern were anslyzed in swine by RT-PCR-RFLP/SSCP. One SNP (a G-->A transition) was identified in exon 12 of PEG1. Maternal expression of PEG1 exon 12 was observed in all major organs (stomach, thymus, pancreas, spleen, lung, muscle, liver, tongue, kidney, brain, bladder, and heart) and placenta of three heterozygous pigs. PEG1 was paternally expressed and maternally imprinted in swine.
Related Concept Videos
Genomic Imprinting and Inheritance
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
Single Nucleotide Polymorphisms-SNPs
Incomplete Dominance
