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Related Experiment Videos

Microbial levan.

Y W Han1

  • 1United States Department of Agriculture, Agricultural Research Service, New Orleans, Louisiana 70179.

Advances in Applied Microbiology
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Levan, a natural fructose polymer, can be produced from sucrose by bacteria. This high-molecular-weight polysaccharide shows promise for industrial applications in food and other sectors.

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Area of Science:

  • Biochemistry
  • Polymer Science
  • Microbiology

Background:

  • Levans are natural fructose polymers found in plants and microbes.
  • Often an unwanted byproduct of sugar processing, levans have potential industrial uses.
  • Production from sucrose offers valuable products from sugarcane juice.

Purpose of the Study:

  • To investigate the production of levan by Bacillus polymyxa.
  • To characterize the structural properties of the synthesized levan.
  • To assess the potential industrial applications of microbial levan.

Main Methods:

  • Cultivation of Bacillus polymyxa (NRRL B-18475) on sucrose solution.
  • Hydrolysis and compositional analysis of the produced polysaccharide.
  • Structural elucidation using 13C-NMR and methylation analysis.

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  • Determination of molecular weight (Mr) and solubility.

    • Main Results:

    • Bacillus polymyxa produced a high yield of polysaccharide from sucrose.
    • The polysaccharide was identified as a beta(2----6)-linked D-fructose polymer.
    • The levan exhibited approximately 12% branching and a Mr of ~2 million.
    • The polymer demonstrated good water solubility.

    Conclusions:

    • Microbial levan production from sucrose is feasible and yields a well-defined polysaccharide.
    • The characterized levan possesses properties suitable for industrial applications.
    • Further development could establish levan as a valuable product in food and other industries.