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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
Forced Transdifferentiation01:28

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

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Updated: May 27, 2026

Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells
06:24

Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells

Published on: October 20, 2023

Reversine increases multipotent human mesenchymal cells differentiation potential.

E Conforti1, E Arrigoni, M Piccoli

  • 1IRCCS Policlinico San Donato, San Donato Milanese, Italy.

Journal of Biological Regulators and Homeostatic Agents
|November 5, 2011
PubMed
Summary
This summary is machine-generated.

Pre-treating human mesenchymal stem cells with reversine significantly boosted their differentiation into various cell types. This chemical approach enhances stem cell plasticity for potential therapeutic applications.

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Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Pharmacology

Background:

  • Adult mesenchymal stem cells (MSCs), including bone marrow-derived (BMSCs) and adipose-derived (ASCs), can differentiate into osteoblasts, adipocytes, and muscle cells.
  • Current limitations in MSC differentiation yields, particularly for skeletal and cardiac muscle, hinder therapeutic applications.
  • Enhancing stem cell plasticity through chemical-pharmacological methods is a promising strategy to improve differentiation efficiency.

Purpose of the Study:

  • To investigate the effect of reversine, a de-differentiation-inducing agent, on the differentiation potential of BMSCs and ASCs.
  • To determine if reversine pre-treatment can improve yields for osteoblast, smooth muscle, and skeletal muscle cell differentiation.
  • To assess the efficacy of reversine at low concentrations for enhancing stem cell differentiation.

Main Methods:

  • Human BMSCs and ASCs were pre-treated with varying concentrations of reversine.
  • Cells were then induced to differentiate into osteoblasts, smooth muscle cells, and skeletal muscle cells.
  • Differentiation yields were quantified and compared between reversine-treated and control groups.

Main Results:

  • Reversine pre-treatment at a low concentration (50 nM) markedly increased differentiation yields for both BMSCs and ASCs.
  • The compound demonstrated efficacy in enhancing differentiation towards osteoblasts, smooth muscle, and skeletal muscle lineages.
  • No significant adverse effects were observed at the tested concentrations.

Conclusions:

  • Low-dose reversine pre-treatment is an effective strategy to enhance the differentiation capacity of human mesenchymal stem cells.
  • This approach holds potential for improving cell-based therapies requiring efficient stem cell differentiation.
  • Further research is warranted to explore the underlying mechanisms and in vivo applications of reversine in regenerative medicine.