Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Growing dune encroachment threatens the habitability of the western Nile riverbank.

Scientific reports·2026
Same author

Retraction Note: Gene therapy with a promoter targeting both rods and cones rescues retinal degeneration caused by AIPL1 mutations.

Gene therapy·2024
Same author

Correction: Development of an optimized AAV2/5 gene therapy vector for Leber congenital amaurosis owing to defects in RPE65.

Gene therapy·2024
Same author

Author Correction: Restoration of vision after transplantation of photoreceptors.

Nature·2024
Same author

Tracking the behavior of an accidental oil spill and its impacts on the marine environment in the Eastern Mediterranean.

Marine pollution bulletin·2023
Same author

Monitoring of oil spill in the offshore zone of the Nile Delta using Sentinel data.

Marine pollution bulletin·2022

Related Experiment Video

Updated: May 27, 2026

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models
06:16

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Published on: March 16, 2022

Ocular gene delivery using lentiviral vectors.

K S Balaggan1, R R Ali

  • 1Department of Genetics, Institute of Ophthalmology, London, UK. kambalaggan@yahoo.co.uk

Gene Therapy
|November 5, 2011
PubMed
Summary

Lentiviral vectors are now safe for clinical trials, offering effective gene delivery for eye diseases. Their ability to target ocular cells stably makes them promising for ophthalmic gene therapy.

Area of Science:

  • Ophthalmology
  • Gene Therapy
  • Virology

Background:

  • Lentivirus research has advanced significantly, enabling the bioengineering of safe lentiviral vectors.
  • These vectors are suitable for clinical trials in various diseases.
  • Ophthalmic gene delivery benefits from lentiviral vectors' properties.

Purpose of the Study:

  • To review the intraocular tropisms and therapeutic applications of lentiviral vectors.
  • To discuss how ocular features influence lentiviral vector performance.
  • To highlight the progress towards human ophthalmic clinical trials.

Main Methods:

  • Review of scientific literature on lentiviral vectors in ocular gene therapy.
  • Analysis of in vivo studies in animal models of ophthalmic disorders.

More Related Videos

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
06:48

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
06:03

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Published on: November 1, 2018

Related Experiment Videos

Last Updated: May 27, 2026

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models
06:16

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Published on: March 16, 2022

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
06:48

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
06:03

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Published on: November 1, 2018

  • Assessment of lentiviral vector bio-safety advancements.

    • Main Results:

    • Lentiviral vectors demonstrate high expression and consistent targeting of ocular cells.
    • They mediate efficient and stable intraocular gene transfer with minimal inflammation.
    • Feasibility established in animal models for anterior and posterior eye diseases.

    Conclusions:

    • Lentiviral vectors are a safe and effective tool for ophthalmic gene therapy.
    • Their unique properties facilitate stable gene transfer in the eye.
    • Progress has led to the initiation of human clinical trials for eye diseases.