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A new, highly sensitive enzymic assay for human tryptase and its use for identification of tryptase inhibitors
S C Dietze1, E A Auerswald, H Fritz
1Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, FRG.
Biological Chemistry Hoppe-Seyler
|May 1, 1990
Abstract:
A chromogenic two-stage assay for human tryptase, a specific marker of mast cell activation, was developed based on the tryptase-induced conversion of prothrombin to thrombin. This assay proved to be more sensitive and reliable than measurements of amidolytic activity of tryptase with small synthetic substrates such as Bz-Arg-Nan and was suitable to detect tryptase activity in human body fluids. In addition, the assay was useful for studies of natural and recombinant inhibitors of tryptase.