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Filifactor alocis interactions with gingival epithelial cells.

C E Moffatt¹, S E Whitmore, A L Griffen

¹Center for Oral Health and Systemic Disease, School of Dentistry, University of Louisville, Louisville, KY 40292, USA.

Molecular Oral Microbiology

|November 8, 2011

Summary

This summary is machine-generated.

Filifactor alocis infection stimulates pro-inflammatory cytokine secretion and induces apoptosis in gingival epithelial cells (GECs). This suggests F. alocis contributes to periodontal tissue destruction, similar to known periodontal pathogens.

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Area of Science:

Oral microbiology
Periodontal disease pathogenesis
Cellular immunology

Background:

Emerging association between Filifactor alocis and periodontal disease.
Limited understanding of F. alocis pathogenic mechanisms.
Need to investigate host-pathogen interactions in periodontal disease.

Purpose of the Study:

To examine the effects of F. alocis infection on primary gingival epithelial cells (GECs).
To elucidate the molecular mechanisms underlying F. alocis-induced cellular responses.
To assess the potential of F. alocis to contribute to periodontal tissue destruction.

Main Methods:

Primary culture of gingival epithelial cells (GECs).
Infection of GECs with F. alocis.
Measurement of pro-inflammatory cytokine secretion (interleukin-1β, interleukin-6, tumor necrosis factor-α).
Assessment of apoptosis and associated signaling pathways (caspase-3, caspase-9, MEK activation).

Main Results:

F. alocis infection stimulated secretion of interleukin-1β, interleukin-6, and tumor necrosis factor-α from GECs.
F. alocis induced apoptosis in GECs via caspase-3 dependent pathways.
Apoptosis was associated with the inhibition of mitogen-activated protein kinase kinase (MEK) activation.
F. alocis demonstrated characteristics similar to established periodontal pathogens.

Conclusions:

F. alocis infection elicits pro-inflammatory responses and induces apoptosis in gingival epithelial cells.
The observed cellular responses suggest a pathogenic role for F. alocis in periodontal disease.
F. alocis has the potential to contribute to periodontal tissue destruction.