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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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Practical observations on the methodology for determining 3-methyl-l-histidine using fluorescamine derivatives
1Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonnington, Nr. Loughborough, Leicestershire LE12 5RD, Great Britain.
Abstract:
The method for the detection and possible quantification of the unusual amino acid 3-methyl-l-histidine has been refined and elucidated. This is an essential prerequisite for the determination of its value as a robust unequivocal index of lean meat protein.
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