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Updated: May 27, 2026

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
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Expression analysis of ADAM17 during mouse eye development.

Saadettin Sel1, Thomas Kalinski, Isabelle Enssen

  • 1Department of Ophthalmology, Martin Luther University Halle/Wittenberg, Halle/Saale, Germany. saadettinsel@googlemail.com

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
|November 8, 2011
PubMed
Summary

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ADAM17 (a disintegrin and metallopeptidase domain 17) is vital for eye development. Its specific expression in ocular tissues during development suggests roles beyond TNF-alpha in eye morphogenesis.

Area of Science:

  • Developmental Biology
  • Ophthalmology
  • Molecular Biology

Background:

  • ADAM17 (a disintegrin and metallopeptidase domain 17) is essential for eye morphogenesis.
  • Understanding its precise expression pattern is key to elucidating its role in ocular development.

Purpose of the Study:

  • To analyze the spatio-temporal expression pattern of ADAM17 during mouse eye development.
  • To investigate the presence of ADAM17 mRNA and protein in ocular tissues at various developmental stages.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) for mRNA detection.
  • DNA sequencing for RT-PCR product verification.
  • Immunohistochemistry for protein localization during embryonic and postnatal development.

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Last Updated: May 27, 2026

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
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Published on: March 15, 2019

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Published on: January 12, 2015

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Main Results:

  • ADAM17 mRNA was detected in adult retina.
  • ADAM17 protein expression was observed in non-pigmented ciliary epithelial cells and retinal vessels from postnatal day 7.
  • Corneal epithelial cells and endothelium showed ADAM17 protein from postnatal day 14.

Conclusions:

  • ADAM17 expression is not ubiquitous throughout the developing eye.
  • The specific expression pattern suggests that ADAM17, along with its substrates beyond TNF-alpha, plays a critical role in eye formation.