Screening method for large numbers of dye-adsorbents for enzyme purification

A method is described by means of which 96 different dye-adsorbents can be tested simultaneously for their ability to bind enzymes and to test their biospecific elution. Small amounts of cell-free extract are applied to dye-adsorbents which are packed in a 96-well transplate cartridge. After biospecific elution, the amount of the eluted enzyme is tested in a microtitre plate assay. The method is illustrated by the purification of glycerol dehydrogenase (E.C. 1.1.1.72), 6-phosphogluconate dehydrogenase (E.C. 1.1.1.44) and glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) from the hyphal fungus Aspergillus nidulans.