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Related Experiment Video

Updated: May 27, 2026

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Allogeneic human tissue-engineered blood vessel.

Clay Quint1, Melissa Arief, Akihito Muto

  • 1Department of Biomedical Engineering, Yale University, New Haven, CT, USA.

Journal of Vascular Surgery
|November 8, 2011
PubMed
Summary
This summary is machine-generated.

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Tissue-engineered vascular grafts (hTEVs) show promise for cardiovascular disease treatment. These decellularized human grafts demonstrated high patency and mechanical stability in a rat model, supporting neointima formation.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Vascular Surgery

Background:

  • Arterial bypass grafting is crucial for advanced cardiovascular disease.
  • A significant unmet need exists for synthetic small-diameter vascular grafts.
  • Current graft options have limitations in long-term efficacy and availability.

Purpose of the Study:

  • To develop and evaluate a decellularized human tissue-engineered vessel (hTEV) as a potential vascular graft.
  • To assess the mechanical properties and in vivo performance of the hTEV.
  • To investigate the host response and regenerative potential of the engineered graft.

Main Methods:

  • Human smooth muscle cells were seeded on biodegradable scaffolds in a perfusion system to create tissue-engineered vessels.

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Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
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Related Experiment Videos

Last Updated: May 27, 2026

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
09:46

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Published on: January 20, 2023

  • A two-step decellularization process using detergents and hypertonic solutions was employed.
  • Mechanical strength (suture retention, burst pressure) and in vivo patency in nude rats were evaluated over six weeks.

    • Main Results:

    • Decellularized hTEVs retained their collagen matrix with intact mechanical strength comparable to human saphenous veins.
    • In vivo implantation in rats showed a high patency rate (4/5 grafts) without rupture or aneurysm over six weeks.
    • Histological analysis revealed a neointima with endothelialization and smooth muscle cell infiltration, along with elastin formation.

    Conclusions:

    • Decellularized human tissue-engineered vessels exhibit promising mechanical stability and patency for arterial grafting.
    • The engineered grafts support host remodeling, including neointima formation with endothelial and smooth muscle cells.
    • These findings suggest hTEVs are a viable regenerative approach for vascular reconstruction.