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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Exogenous intravascular nitric oxide enhances ventricular function after hemodilution with plasma expander
Surapong Chatpun1, Pedro Cabrales
1Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093-0412, USA.
Life Sciences
|November 8, 2011
Summary
Nitric oxide (NO) supplementation during acute hemodilution with plasma expanders improves cardiac function and vasodilation. However, these beneficial effects are dose-independent and short-lived.
Area of Science:
- Cardiovascular Physiology
- Pharmacology
Background:
- Acute hemodilution with plasma expanders can impact cardiac function.
- Nitric oxide (NO) plays a crucial role in regulating vascular tone and cardiac performance.
Purpose of the Study:
- To evaluate the hypothesis that exogenous nitric oxide (NO) supplementation during acute hemodilution with plasma expander (PE) benefits cardiac function.
- To investigate the effects of a NO donor on cardiac performance and systemic vascular resistance following hemodilution.
Main Methods:
- Acute hemodilution was induced in hamsters by exchanging 40% of blood volume with dextran.
- Intravascular NO supplementation was achieved using diethylenetriamine NONOate (DETA NONOate) after hemodilution.
- Left ventricular cardiac function was assessed using pressure-volume measurements, and microvessel vasodilation was analyzed via intravital microscopy.
Main Results:
- NO supplementation increased cardiac output and significantly reduced stroke work per stroke volume (SW/SV) and systemic vascular resistance (SVR) compared to vehicle.
- The NO donor group exhibited significantly lower minimum rate of pressure change (dP/dt(min)) than the vehicle group.
- Intravital microscopy confirmed significant vasodilation in the NO donor group compared to the vehicle group.
Conclusions:
- Exogenous NO supplementation during acute hemodilution with PE demonstrates beneficial effects on cardiac performance and systemic vascular resistance.
- The observed effects of NO supplementation using a NO donor were found to be dose-independent and transient.
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