Related Experiment Video
Updated: May 27, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Cyclic bisdesmosides from Actinostemma lobatum MAXIM (Cucurbitaceae) and their in vitro cytotoxicity
Wei Li1, Jiaqing Cao, Yun Tang
1Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Abstract:
Two new cyclic bisdesmosides elucidated as lobatoside L (1) and lobatoside M (2) and four known cyclic bisdesmosides (3-6) were isolated from Actinostemma lobatum MAXIM (Cucurbitaceae). Their structures were determined on the basis of spectroscopic analyses including IR, HR-FAB-MS, TOCSY, 1D- and 2D-NMR experiments and chemical reactions. The inhibitory effects of the six compounds on human cancer cell growth (including esophageal squamous carcinoma cell line ECA109, lung cancer cell line A549 and gastric cancer cell line MGC-803) were determined using the MTT assay. The results revealed that the six compounds exhibited cytotoxicity against all the cell lines tested, and compounds 1, 3 and 5 showed significant activities in a dose dependent manner against all the cell lines, especially for compound 1 and 5, the IC(50) values for ECA-109 cells were 8.25 μM and 3.71 μM. The results demonstrated that compounds 1 and 5's activities are 2- and 4-fold that of cisplatin.
More Related Videos
13:17In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer Photopharmacology
Published on: September 29, 2023
14:33Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
Published on: April 8, 2022
Related Concept Videos
Drugs that Destabilize Microtubules
Inhibition of Cdk Activity
Drugs that Stabilize Microtubules
Antifungal Agents
Inhibitors of Bacterial DNA Synthesis