Related Experiment Video
Updated: May 27, 2026

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
Published on: February 25, 2016
Cell cycle-dependent expression and subcellular localization of fructose 1,6-bisphosphatase
Piotr Mamczur1, Agnieszka Joanna Sok, Adam Rzechonek
1Department of Animal Molecular Physiology, Institute of Zoology, Wrocław University, Cybulskiego 30, 50-205 Wrocław, Poland.
Abstract:
Recently a gluconeogenic enzyme was discovered-fructose 1,6-bisphosphatase (FBPase)-that localizes in the nucleus of a proliferating cell, but its physiological role in this compartment remains unclear. Here, we demonstrate the link between nuclear localization of FBPase and the cell cycle progression. Results of our studies indicate that in human and mouse squamous cell lung cancer, as well as in the HL-1 cardiomyocytes, FBPase nuclear localization correlates with nuclear localization of S and G2 phase cyclins. Additionally, activity and expression of the enzyme depends on cell cycle stages. Identification of FBPase interacting partners with mass spectrometry reveals a set of nuclear proteins involved in cell cycle regulation, mRNA processing and in stabilization of genomic DNA structure. To our knowledge, this is the first experimental evidence that muscle FBPase is involved in cell cycle events.
Related Concept Videos
Cell Specific Gene Expression
MAPK Signaling Cascades
Positive Regulator Molecules
Positive Regulator Molecules
Cells Coordinate Growth and Proliferation
Molecular Factors Affecting Cell Division
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

