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Updated: May 27, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

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Glycoarray by DNA-directed immobilization.

François Morvan¹, Yann Chevolot, Jing Zhang

¹Institut des Biomoléules Max Mousseron, UMR 5247, CNRS Université Montpellier 1, Université Montpellier 2, Montpellier, France.

Methods in Molecular Biology (Clifton, N.J.)

|November 8, 2011

Summary

This study presents a new method for creating carbohydrate microarrays using oligonucleotide glycomimetic conjugates and DNA-directed immobilization (DDI). This efficient technique enables precise carbohydrate placement on surfaces for biological interaction studies.

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Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

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Published on: February 5, 2018

Area of Science:

Carbohydrate chemistry
Bioconjugation
Microarray technology

Background:

Glycoarrays are essential tools for studying carbohydrate-mediated biological events.
Effective immobilization of carbohydrates is crucial for glycoarray fabrication.
Existing immobilization strategies have limitations in efficiency and selectivity.

Purpose of the Study:

To develop a protocol for synthesizing oligonucleotide glycomimetic conjugates.
To establish a method for immobilizing these conjugates onto DNA microarrays using DNA-directed immobilization (DDI).
To enable the creation of precisely patterned carbohydrate microarrays.

Main Methods:

Synthesis of fluorescent oligonucleotide trigalactosylmimetic conjugates.
Utilizing DNA-directed immobilization (DDI) for site-selective attachment.
Fabrication of carbohydrate microarrays on glass slides.

Main Results:

Successful synthesis of oligonucleotide glycomimetic conjugates.
Demonstration of efficient and site-selective immobilization via DDI.
Generation of functional carbohydrate microarrays with precise patterning.

Conclusions:

The proposed protocol offers a robust method for preparing oligonucleotide glycomimetic conjugates.
DDI provides an efficient and selective approach for carbohydrate microarray fabrication.
This technique facilitates advanced studies of carbohydrate-protein interactions.