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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 27, 2026

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins
10:32

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

Published on: May 29, 2016

Probing virus-glycan interactions using glycan microarrays.

Jamie Heimburg-Molinaro1, Mary Tappert, Xuezheng Song

  • 1Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 8, 2011
PubMed
Summary

This study details methods for using glycan microarrays to analyze influenza virus interactions with specific glycans. These protocols enable better understanding of virus-glycan binding crucial for pathogenesis.

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Last Updated: May 27, 2026

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins
10:32

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Published on: May 29, 2016

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Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Area of Science:

  • Biochemistry
  • Virology
  • Immunology

Background:

  • Glycan microarrays immobilize glycans to study interactions with proteins.
  • Glycan-binding proteins (GBPs) on viruses are key to pathogenesis.
  • Understanding virus-glycan interactions is vital for antiviral strategies.

Purpose of the Study:

  • To provide detailed protocols for studying influenza virus-glycan interactions using glycan microarrays.
  • To establish a standardized methodology for analyzing these binding events.
  • To facilitate research into viral pathogenesis and host-pathogen interactions.

Main Methods:

  • Fluorescent labeling of influenza virus particles.
  • Application of labeled virus to a glycan microarray containing immobilized glycans.
  • Image acquisition and analysis of glycan microarray slides.
  • Data interpretation to identify specific glycan binding specificities.

Main Results:

  • The described protocols allow for the identification of specific glycans that bind to influenza viruses.
  • Quantification of virus binding to individual glycans on the microarray.
  • Characterization of the glycan-binding profile of influenza virus strains.

Conclusions:

  • Glycan microarrays are powerful tools for dissecting virus-glycan interactions.
  • These protocols provide a reproducible method for studying influenza virus tropism and pathogenesis.
  • The findings can inform the development of novel antiviral therapies and vaccines.