Use of a calcium-sensitive electrode for studies on mitochondrial calcium transport

Ca(2+)-sensitive electrode as a practical approach is used to follow Ca(2+) changes in the medium and particularly useful to study mitochondrial Ca(2+) uptake (or release); this method permits the continuous recording of Ca(2+) movements through the mitochondrial inner membrane. In this chapter, it is described how to prepare a Ca(2+)-sensitive electrode, and its application on mitochondrial studies with emphasis on the mitochondrial permeability transition.