Jove
Visualize
Contact Us

Related Concept Videos

Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biotechnological advances in torularhodin production: artificial neural networks as a tool for improving and biocompatibility studies.

Preparative biochemistry & biotechnology·2025
Same author

Assessment of artificial neural networks to predict red colorant production by Talaromyces amestolkiae.

Bioprocess and biosystems engineering·2022
Same author

Treatment of real dairy wastewater by electrolysis and photo-assisted electrolysis in presence of chlorides.

Water science and technology : a journal of the International Association on Water Pollution Research·2019
Same author

Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides.

Applied biochemistry and biotechnology·2019
Same author

Electrochemical production and use of free chlorine for pollutant removal: an experimental design approach.

Environmental technology·2017
Same author

Experimental-design-guided approach for the removal of atrazine by sono-electrochemical-UV-chlorine techniques.

Environmental technology·2017
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 27, 2026

OnePot PURE Cell-Free System
08:25

OnePot PURE Cell-Free System

Published on: June 23, 2021

Preliminary studies for cephamycin C purification technique.

Alvaro de Baptista Neto1, Maritza Catalina Condori Bustamante, Jaine Honorata Hortolan Luiz de Oliveira

  • 1Verdartis Desenvolvimento Biotecnológico Ltda-ME, Rua dos Técnicos s/n sala 5, Incubadora Supera Campus USP, Riberão Preto, SP, Brazil.

Applied Biochemistry and Biotechnology
|November 8, 2011
PubMed
Summary
This summary is machine-generated.

This study details the purification of cephamycin C from Streptomyces clavuligerus fermentation using filtration and chromatography. The methods successfully isolated and identified the antibiotic, proving the process feasibility.

More Related Videos

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
08:48

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Published on: July 11, 2025

Related Experiment Videos

Last Updated: May 27, 2026

OnePot PURE Cell-Free System
08:25

OnePot PURE Cell-Free System

Published on: June 23, 2021

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
08:48

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Published on: July 11, 2025

Area of Science:

  • Biotechnology
  • Microbiology
  • Pharmaceutical Sciences

Background:

  • Cephamycin C is a vital antibiotic produced by Streptomyces clavuligerus.
  • Efficient purification methods are crucial for obtaining high-purity cephamycin C for therapeutic use.

Purpose of the Study:

  • To develop and assess a feasible purification process for cephamycin C from Streptomyces clavuligerus fermentation broth.
  • To evaluate the effectiveness of various purification techniques, including filtration and chromatography.

Main Methods:

  • Clarification of fermentation broth using microfiltration and ultrafiltration.
  • Purification of permeates via nonspecific adsorption and ion-exchange chromatography on resin columns.
  • Antibiotic activity assay against Escherichia coli ESS, UV spectrophotometry, NMR spectroscopy, and mass spectrometry (LC/MS, LC/MS/MS) for analysis and identification.

Main Results:

  • Successful clarification and purification of cephamycin C from the fermentation broth.
  • Demonstrated feasibility of combining filtration, nonspecific adsorption, and ion-exchange chromatography for antibiotic purification.
  • Identification of cephamycin C molecule in purified fractions using advanced mass spectrometry techniques.

Conclusions:

  • The integrated purification strategy involving filtration and chromatography is effective for isolating cephamycin C.
  • The study validates a robust process for obtaining purified cephamycin C, suitable for further development.
  • Preliminary results confirm the feasibility and potential of the described purification methodology.