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Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
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Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...
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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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5-O-Acetyl-d-ribono-1,4-lactone.

Adailton J Bortoluzzi1, Damianni Sebrão, Marcus M Sá

  • 1Depto. de Química - UFSC, 88040-900 - Florianópolis, SC, Brazil.

Acta Crystallographica. Section E, Structure Reports Online
|November 8, 2011
PubMed
Summary

Enzyme-catalyzed acylation of d-ribono-1,4-lactone yielded a novel compound. Its crystal structure reveals an envelope conformation and a 1D polymer formed by hydrogen bonds.

Area of Science:

  • Carbohydrate Chemistry
  • Crystallography
  • Enzymology

Background:

  • d-ribono-1,4-lactone is a key carbohydrate derivative.
  • Enzyme-catalyzed reactions offer high selectivity in organic synthesis.
  • Understanding molecular conformations and crystal packing is crucial in structural chemistry.

Purpose of the Study:

  • To synthesize and characterize a novel acylated derivative of d-ribono-1,4-lactone.
  • To elucidate the molecular structure, including ring conformation and intermolecular interactions.
  • To investigate the crystal packing and dimensionality of the synthesized compound.

Main Methods:

  • Regioselective enzyme-catalyzed acylation reaction.
  • Single-crystal X-ray diffraction analysis.

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  • Conformational analysis and crystal structure determination.

    • Main Results:

    • Successful synthesis of the title compound, C(7)H(10)O(6), via regioselective enzymatic acylation.
    • The five-membered ring of the acylated sugar adopts an envelope conformation.
    • Crystal structure analysis revealed a one-dimensional polymeric chain formed by intermolecular O-H⋯O hydrogen bonds, with stacking along the b axis.

    Conclusions:

    • The study successfully synthesized and characterized a novel acylated d-ribono-1,4-lactone derivative.
    • The compound exhibits a distinct envelope conformation and forms a 1D polymer in the solid state.
    • Enzymatic acylation provides a regioselective route to complex carbohydrate derivatives with interesting structural features.