Related Experiment Video
Updated: May 27, 2026

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Study of proteolysis during the processing of a dry fermented pork sausage
G D García de Fernando1, P F Fox
1Department of Food Chemistry, University College, Cork, Republic of Ireland.
Abstract:
Dry pork sausage was formulated, fermented and dried for 41 days. Gross composition was determined and proteolysis was monitored by assaying the following N fractions: water-soluble nitrogen (WSN), salt-soluble nitrogen (SSN), ultrafiltration (UF) permeates of WSN and SSN, phosphotungstic acid (PTA)-soluble and -insoluble fractions and free amino acids. Meat and sausages, water- and salt-insoluble components, UF-retentates of WSN and SSN were assessed by SDS-PAGE at different stages of ripening. The amount of WSN, WSN permeate, PTA-soluble N and free amino acids increased during processing, while the SSN and PTA-insoluble N decreased. The electrophoretic studies demonstrated that proteolysis of the heavy myosin chain, α-actinin and actin was most prominent. The increased insolubility of meat proteins was confirmed by SDS-PAGE.
Related Concept Videos
Protein Digestion
The Proteasome Structure
The proteasome is an...
Microbes in the Production of Fermented Foods
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...

