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Survey: immune function and immunotoxicity assessment in dogs.
Hervé Lebrec1, Raegan O'Lone, Wendy Freebern
1Comparative Biology and Safety Sciences, Amgen Inc., Seattle, WA 98119-3105, USA. hlebrec@amgen.com
Journal of Immunotoxicology
|November 9, 2011
Summary
Immunology and immunotoxicity assessments in dogs are necessary for research and toxicology. A survey revealed available assays, gaps, and the need for better immunophenotyping reagents in canine models.
Area of Science:
- Veterinary immunology
- Toxicology
- Drug development
Background:
- Standard toxicology studies sometimes require canine immunotoxicology evaluations.
- The HESI Immunotoxicology Technical Committee surveyed current practices.
Purpose of the Study:
- To assess the availability and necessity of immunology and immunotoxicity assays in dogs.
- To identify gaps and needs in canine immunotoxicology testing.
Main Methods:
- An email survey was distributed to scientists in industry and academia.
- Fifteen institutions (pharmaceutical, biotech, CROs, academic) responded.
Main Results:
- Canine immunological assessments are deemed necessary for research and toxicology.
- Various assays are used, including antibody responses, immunoglobulins, complement CH(50), cytokines, and cell proliferation.
- Gaps exist in complement components, NK and T-cell immunophenotyping, and optimization is needed for certain functional assays.
Conclusions:
- Canine models are essential for specific immunological assessments in drug development.
- Further development of assays and reagents is required to fully characterize canine immune responses.
- Enhanced understanding of immunophenotyping reagent specificity is needed.
