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Updated: May 27, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
The minimum quantity of nitrite required to stain sliced and homogenised cooked pork
D B Macdougall1, M J Hetherington
1A.F.R.C., Institute of Food Research, Reading Laboratory, Shinfield, Reading, RG2 9AT, UK.
Abstract:
The levels of nitrite used in curing pork are in excess of those required to produce an adequate cured colour, but the minimum level for pink coloration to be visible has not been established experimentally. This paper demonstrates the effect of low nitrite levels from local surface application to pork muscle, simulating inadvertent contamination from work surfaces. An homogenate is used for comparison in which the nitrite was evenly distributed throughout the product. The results indicate that surface contact with nitrite produces essentially the same pattern of colour change as in the homogenate. Visible pink coloration was evident from 1-2 mg kg(-1) sodium nitrite absorbed.
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