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Updated: May 27, 2026
Development and Standardization of an Ex Vivo Micromethod for Intracellular Quantification of Vincristine in Primary ALL Cells by LC-MS/MS
Published on: January 23, 2026
A sensitive liquid chromatography coupled with mass spectrometry method for the intracellular and plasma
Stéphanie Mosnier-Thoumas1, Sarah Djabarouti, Fabien Xuereb
1Department of Clinical Pharmacokinetics EA 2968, Clinical Pharmacy Haut-Lévêque Hospital CHU Bordeaux, University of Bordeaux 2, Pessac, France.
Introduction:
Liquid chromatography coupled with mass spectrometry for the quantification of raltegravir in human plasma and peripheral blood mononuclear cells has been developed.
Methods:
Sample preparations were based on a fully automated solid-phase extraction process. Mass spectrometric data were acquired in a single-ion monitoring method. Raltegravir and quinoxaline, the internal standard, were well separated in a gradient mode over 15 min.
Key Findings:
Validation study exhibited excellent linearity, with good intra- and inter-day precision and accuracy.
Conclusions:
The assay was successfully applied to the raltegravir quantification in HIV-infected patients.
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