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Serum skatole detection using gas chromatography and high performance liquid chromatography
R S Lin1, M W Orcutt, J A Patterson
1Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Meat Science
|November 9, 2011
Summary
Frozen storage did not degrade skatole in boar serum. Gas chromatography (GC) and high-performance liquid chromatography (HPLC) accurately measured skatole levels, showing no significant differences between fresh and stored samples.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Food Science
Background:
- Skatole is a compound found in animal products, and its accurate measurement is crucial for quality control.
- Understanding skatole stability during storage is essential for reliable analytical results.
Purpose of the Study:
- To assess the degradation of skatole in boar serum during frozen storage.
- To compare the efficacy of gas chromatography (GC) and high-performance liquid chromatography (HPLC) for quantifying serum skatole.
Main Methods:
- Boar serum was spiked with 1 ppm skatole.
- Samples were subjected to frozen storage at -15°C for 4 months.
- Skatole concentrations were measured using both GC and HPLC.
Main Results:
- No significant differences in skatole recovery or sensitivity were found between GC and HPLC.
- Measurements obtained by GC and HPLC showed a high correlation (r = 0.93).
- No statistically significant degradation of skatole was observed in serum stored for 4 months at -15°C compared to fresh samples.
Conclusions:
- Frozen storage at -15°C for 4 months does not significantly affect skatole levels in boar serum.
- Both GC and HPLC are reliable and comparable methods for measuring skatole in boar serum, even after frozen storage.
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