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Arcobacter spp. enumeration in poultry meat using a combined PCR-ELISA assay
A Antolín1, I González, T García
1Departamento de Nutrición y Bromatologia III (Higiene y Tecnologı́a de los Alimentos), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Meat Science
|November 9, 2011
Summary
A new assay rapidly detects Arcobacter bacteria in chicken meat using polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). This method allows for accurate quantification of Arcobacter spp. in poultry products.
Area of Science:
- Food Microbiology
- Molecular Biology
- Diagnostic Assays
Background:
- Arcobacter species are emerging foodborne pathogens found in poultry.
- Accurate enumeration of Arcobacter in food matrices is crucial for food safety.
- Existing methods for Arcobacter detection can be time-consuming.
Purpose of the Study:
- To develop a rapid and sensitive assay for enumerating Arcobacter spp. in chicken meat.
- To combine molecular amplification with a colorimetric detection system.
Main Methods:
- Development of a novel assay integrating selective enrichment, DNA extraction, and PCR amplification.
- Utilized digoxigenin-labelled primers specific for Arcobacter 16S rRNA genes.
- Quantification via enzyme-linked immunosorbent assay (ELISA) using a digoxigenin-specific conjugate.
Main Results:
- The assay successfully detected and quantified Arcobacter spp. in chicken meat samples.
- Distinct absorbance differences were observed for samples containing Arcobacter spp. within the range of 10-10(4) CFU/g.
- The assay demonstrated sensitivity and specificity for Arcobacter enumeration.
Conclusions:
- The developed PCR-ELISA assay provides a rapid and reliable method for Arcobacter spp. enumeration in poultry.
- This assay can significantly improve the monitoring of Arcobacter contamination in chicken meat.
- The technique offers a valuable tool for food safety laboratories and regulatory agencies.
