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Updated: May 27, 2026

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Analysis of raw meat to predict proteolysis in Parma ham
C Schivazappa1, M Degni, L Nanni Costa
1Stazione Sperimentale per l'Industria delle Conserve Alimentari, V.le F. Tanara 31/A, 43100 Parma, Italy.
Abstract:
Four hundred and thirty-seven pigs (223 purebred Italian Large White, 97 Italian Landrace, and 117 Duroc), were studied to examine the effect of breed on meat quality and assess the possibility of relating proteolysis of dry-cured hams to raw meat quality. The Duroc pigs had intramuscular fat contents and water holding capacities (M. Semimembranosus) significantly higher than those of the Large White and Landrace. The latter had a significantly higher pH(24h) and cathepsin B activities significantly lower than the Duroc breed. The dry-cured hams (M. Biceps femoris) from the three breeds were significantly different in proximate composition, proteolysis and weight loss at the end of ageing. Data for green hams (including salt content) were used to compute a model to fit the proteolysis of the corresponding dry-cured hams. The variables included in the model (R(2)=0.53 and P<0.01) were cathepsin B activity of raw ham, pH(24h), weight loss after the first salting step, and the salt content of the dry-cured ham. The raw hams with the highest cathepsin B activities, the lowest pH(24h), and the highest weight loss after the first salting were those in which greatest proteolysis occured.

